The prevalence of Set1 A and Set1 B genes in clinical Shigella sonnei strains using multiplex-PCR

Sadeghifard, M. and Amini, K. and Nasr, J. and Yahyaraeyat, R. (2016) The prevalence of Set1 A and Set1 B genes in clinical Shigella sonnei strains using multiplex-PCR. Feyz Journal of Kashan University of Medical Sciences, 20.

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Official URL: http://feyz.kaums.ac.ir/article-1-3175-en.html

Abstract

Background: Shigella spp have been reported as the common cause of mortality in children. Recent studies in Iran have shown that Shigella sonnei is an important specie for nosocomial infection. The set1A and set1B are two critical virulence factors for human disease. The aim of this study was to evaluate the frequency of set1A and set1B genes in bacterial strains isolated from people with diarrhea dysentery using the multiplex-PCR (M-PCR) method. Materials and Methods: A total 60 strains of Shigella sonnei were collected from Children Medical Center (Tehran, Iran). Then, these isolates were identified by standard biochemical and culture phenotypic methods. Detection of both of set1B and set1A virulence genes was carried out by M-PCR. Antibacterial susceptibility testing to Amoxicillin, Clavulanic acid, Tetracycline, Cefixime, Ceftriaxone, Cefepime and Cotrimoxazole was performed according to CLSI guideline using disk diffusion method. Results: All 60 isolates were identified as S. sonnei. The highest resistance was observed to both of Cotrimoxazole and Cefixime antibiotics, while 95 of strains were sensitive to Tetracycline. The prevalence of Set1A and set1B virulence genes were 18 (30) and 0 (0) strains, respectively. Conclusion: Nowadays, the increase of microbial resistance to antibiotics is a global problem which is caused by indiscriminate use of drugs. The results showed that the prevalence of the gene in the sample set could be a criterion for direct detection of Shigella in the sample.

Item Type: Article
Subjects: Immunology and Microbiology
Divisions: Feyz journal
Depositing User: ART . editor
Date Deposited: 06 May 2017 15:04
Last Modified: 12 Jun 2017 11:56
URI: http://eprints.kaums.ac.ir/id/eprint/1453

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